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MVP/LRP Polyclonal antibody
MVP/LRP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 16478-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, RAW264.7, human brain tissue, mouse lung tissue, rat lung tissue, SH-SY5Y cells |
Positive IP detected in | mouse lung tissue |
Positive IHC detected in | human breast cancer tissue, human testis tissue, human skin tissue, human lung cancer tissue, human kidney tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 9 publications below |
IHC | See 3 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
16478-1-AP targets MVP/LRP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MVP/LRP fusion protein Ag9593 相同性解析による交差性が予測される生物種 |
Full Name | major vault protein |
Calculated molecular weight | 893 aa, 99 kDa |
Observed molecular weight | 99-104 kDa |
GenBank accession number | BC015623 |
Gene symbol | LRP |
Gene ID (NCBI) | 9961 |
RRID | AB_2147597 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Vaults are the largest cellular ribonuclear protein complexes with a hollow barrel-like structure, and have been associated with the MDR phenotype. Major vault protein (MVP) is the main component of vaults and is presumed to be involved in MDR. MVP is identical with the human lung resistance protein (LRP), known to be overexpressed in multiple chemotherapy resistance models [PMID:23083532]. MVP has been associated to resistance to radiotherapy [4], probably due to its role in preventing apoptosis by inhibiting the COP-1/p53 axis [PMID: 12894554, 15994960].
Protocols
Product Specific Protocols | |
---|---|
WB protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
IHC protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
IF protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
IP protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. | ||
Drug Resist Updat CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis
| ||
Nat Commun MVP-mediated exosomal sorting of miR-193a promotes colon cancer progression.
| ||
Front Cell Dev Biol Structural and Functional Characterization of Fibronectin in Extracellular Vesicles From Hepatocytes. | ||
Front Cell Dev Biol Major Vault Protein (MVP) Associated With BRAF V600E Mutation Is an Immune Microenvironment-Related Biomarker Promoting the Progression of Papillary Thyroid Cancer via MAPK/ERK and PI3K/AKT Pathways.
| ||
Cell Commun Signal Differential RNA packaging into small extracellular vesicles by neurons and astrocytes. |