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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MVP/LRP Polyclonal antibody

MVP/LRP Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16478

Synonyms

LRP, major vault protein, MVP, MVP/LRP, VAULT1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated MVP/LRP Polyclonal a (CL488-16478)

    IF Staining of HepG2 using CL488-16478

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 MVP/LRP antibody (CL488-16478) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated MVP/LRP Polyclonal a (CL488-16478)

    FC experiment of HepG2 using CL488-16478

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 MVP/LRP Polyclonal Antibody (CL488-16478)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16478 targets MVP/LRP in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MVP/LRP fusion protein Ag9593 相同性解析による交差性が予測される生物種
Full Name major vault protein
Calculated molecular weight 893 aa, 99 kDa
Observed molecular weight 99-104 kDa
GenBank accession numberBC015623
Gene symbol LRP
Gene ID (NCBI) 9961
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vaults are the largest cellular ribonuclear protein complexes with a hollow barrel-like structure, and have been associated with the MDR phenotype. Major vault protein (MVP) is the main component of vaults and is presumed to be involved in MDR. MVP is identical with the human lung resistance protein (LRP), known to be overexpressed in multiple chemotherapy resistance models [PMID:23083532]. MVP has been associated to resistance to radiotherapy [4], probably due to its role in preventing apoptosis by inhibiting the COP-1/p53 axis [PMID: 12894554, 15994960].

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MVP/LRP antibody CL488-16478Download protocol
FC protocol for CL Plus 488 MVP/LRP antibody CL488-16478Download protocol
Standard Protocols
Click here to view our Standard Protocols