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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MX1 Recombinant antibody (82677-1-RR)

    WB analysis using 82677-1-RR (same clone as 82677-1-PBS)

    Untreated and IFN alpha treated A549 cells, untreated and IFN alpha treated HepG2 cells were subjected to SDS PAGE followed by western blot with 82677-1-RR (MX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MX1 Recombinant antibody (82677-1-RR)

    IHC staining of human tonsillitis using 82677-1-RR (same clone as 82677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MX1 Recombinant antibody (82677-1-RR)

    IHC staining of human lung cancer using 82677-1-RR (same clone as 82677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MX1 Recombinant antibody (82677-1-RR)

    IHC staining of human lung cancer using 82677-1-RR (same clone as 82677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MX1 Recombinant antibody (82677-1-RR)

    IHC staining of human tonsillitis using 82677-1-RR (same clone as 82677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MX1 Recombinant antibody (82677-1-RR)

    IF Staining of A549 using 82677-1-RR (same clone as 82677-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MX1 antibody (82677-1-RR, Clone: 2L19 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82677-1-RR

    Affinity and Kinetic Characterization of 82677-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82677-1-RR against Human MX1 were performed. The affinity constant is 0.763 nM.

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MX1 Recombinant antibody, PBS Only

MX1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82677-1-PBS
Clone No.2L19

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Interferon-regulated resistance GTP-binding protein MxA, Interferon-induced protein p78, Interferon-induced GTP-binding protein Mx1, N-terminally processed, Interferon-induced GTP-binding protein Mx1, IFI-78K

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82677-1-PBS targets MX1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MX1 fusion protein Ag4692 相同性解析による交差性が予測される生物種
Full Name Myxovirus resistance protein 1
Calculated molecular weight 662 aa, 76 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC032602
Gene Symbol MX1
Gene ID (NCBI) 4599
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20591
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN.