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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MX1 Recombinant antibody

MX1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2L19

Cat no : 82677-1-RR

Synonyms

Interferon-regulated resistance GTP-binding protein MxA, Interferon-induced protein p78, Interferon-induced GTP-binding protein Mx1, N-terminally processed, Interferon-induced GTP-binding protein Mx1, IFI-78K

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MX1 Recombinant antibody (82677-1-RR)

    WB analysis using 82677-1-RR

    Untreated and IFN alpha treated A549 cells, untreated and IFN alpha treated HepG2 cells were subjected to SDS PAGE followed by western blot with 82677-1-RR (MX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MX1 Recombinant antibody (82677-1-RR)

    IHC staining of human lung cancer using 82677-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MX1 Recombinant antibody (82677-1-RR)

    IHC staining of human lung cancer using 82677-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MX1 Recombinant antibody (82677-1-RR)

    IHC staining of human tonsillitis using 82677-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MX1 Recombinant antibody (82677-1-RR)

    IHC staining of human tonsillitis using 82677-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MX1 Recombinant antibody (82677-1-RR)

    IF Staining of A549 using 82677-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MX1 antibody (82677-1-RR, Clone: 2L19 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82677-1-RR

    Affinity and Kinetic Characterization of 82677-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82677-1-RR against Human MX1 were performed. The affinity constant is 0.763 nM.

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Tested Applications

Positive WB detected inIFN alpha treated A549 cells, IFN alpha treated HepG2 cells
Positive IHC detected inhuman lung cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82677-1-RR targets MX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MX1 fusion protein Ag4692 相同性解析による交差性が予測される生物種
Full Name Myxovirus resistance protein 1
Calculated molecular weight 662 aa, 76 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC032602
Gene symbol MX1
Gene ID (NCBI) 4599
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN.

Protocols

Product Specific Protocols
WB protocol for MX1 antibody 82677-1-RRDownload protocol
IHC protocol for MX1 antibody 82677-1-RRDownload protocol
IF protocol for MX1 antibody 82677-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols