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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYB/c-Myb Polyclonal antibody, PBS Only

MYB/c-Myb Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17800-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYB/c-Myb Polyclonal antibody (17800-1-AP)

    WB analysis using 17800-1-AP (same clone as 17800-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17800-1-AP (MYB/c-Myb antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17800-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MYB/c-Myb Polyclonal antibody (17800-1-AP)

    WB analysis using 17800-1-AP (same clone as 17800-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17800-1-AP (MYB/c-Myb antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17800-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MYB/c-Myb Polyclonal antibody (17800-1-AP)

    WB analysis of Jurkat using 17800-1-AP (same clone as 17800-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17800-1-AP (MYB/c-Myb antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17800-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HL-60 cells using MYB/c-Myb Polyclonal antibody (17800-1-AP)

    IP experiment of HL-60 using 17800-1-AP (same clone as 17800-1-PBS)

    IP result of anti-MYB/c-Myb (IP:17800-1-AP, 4ug; Detection:17800-1-AP 1:300) with HL-60 cells lysate 4000ug. This data was developed using the same antibody clone with 17800-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17800-1-PBS targets MYB/c-Myb in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYB/c-Myb fusion protein Ag12021 相同性解析による交差性が予測される生物種
Full Name v-myb myeloblastosis viral oncogene homolog (avian)
Calculated molecular weight 640 aa, 72 kDa
Observed molecular weight40-50 kDa, 72-80 kDa
GenBank accession numberBC064955
Gene symbol c-Myb
Gene ID (NCBI) 4602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.