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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYBBP1A Monoclonal antibody (67996-1-Ig)

    WB analysis using 67996-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using MYBBP1A Monoclonal antibody (67996-1-Ig)

    WB analysis of HSC-T6 using 67996-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using MYBBP1A Monoclonal antibody (67996-1-Ig)

    WB analysis of NIH/3T3 using 67996-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using MYBBP1A Monoclonal antibody (67996-1-Ig)

    WB analysis of 4T1 using 67996-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MYBBP1A Monoclonal antibody (67996-1-Ig)

    IHC staining of human kidney using 67996-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67996-1-Ig (MYBBP1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using MYBBP1A Monoclonal antibody (67996-1-Ig)

    IF Staining of NIH/3T3 using 67996-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using MYBBP1A Monoclonal antibody (67996-1-Ig)

    IF Staining of NIH/3T3 using 67996-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYBBP1A Monoclonal antibody (67996-1-Ig)

    IF Staining of HepG2 using 67996-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYBBP1A Monoclonal antibody (67996-1-Ig)

    IF Staining of HepG2 using 67996-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

［PR］論文掲載5,600報突破！GFP免疫沈降用抗体「GFP-Trap®」を無料体験

MYBBP1A Monoclonal antibody

MYBBP1A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67996-1-Ig
Clone No.1H2D10

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FLJ37886, MYB binding protein (P160) 1a, Myb binding protein 1A, MYBBP1A, P160, PAP2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67996-1-Ig targets MYBBP1A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYBBP1A fusion protein Ag6008 相同性解析による交差性が予測される生物種
Full Name MYB binding protein (P160) 1a
Calculated molecular weight 149 kDa
Observed molecular weight140 kDa
GenBank accession numberBC050546
Gene Symbol MYBBP1A
Gene ID (NCBI) 10514
RRIDAB_2918745
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BQG0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996].

Protocols

Product Specific Protocols
WB protocol for MYBBP1A antibody 67996-1-IgDownload protocol
IHC protocol for MYBBP1A antibody 67996-1-IgDownload protocol
IF protocol for MYBBP1A antibody 67996-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols