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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYBBP1A Monoclonal antibody (67996-1-Ig)

    WB analysis using 67996-1-Ig (same clone as 67996-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using MYBBP1A Monoclonal antibody (67996-1-Ig)

    WB analysis of HSC-T6 using 67996-1-Ig (same clone as 67996-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using MYBBP1A Monoclonal antibody (67996-1-Ig)

    WB analysis of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using MYBBP1A Monoclonal antibody (67996-1-Ig)

    WB analysis of 4T1 using 67996-1-Ig (same clone as 67996-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MYBBP1A Monoclonal antibody (67996-1-Ig)

    IHC staining of human kidney using 67996-1-Ig (same clone as 67996-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67996-1-Ig (MYBBP1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using MYBBP1A Monoclonal antibody (67996-1-Ig)

    IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using MYBBP1A Monoclonal antibody (67996-1-Ig)

    IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYBBP1A Monoclonal antibody (67996-1-Ig)

    IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYBBP1A Monoclonal antibody (67996-1-Ig)

    IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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MYBBP1A Monoclonal antibody, PBS Only

MYBBP1A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67996-1-PBS
Clone No.1H2D10

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FLJ37886, MYB binding protein (P160) 1a, Myb binding protein 1A, MYBBP1A, P160, PAP2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67996-1-PBS targets MYBBP1A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYBBP1A fusion protein Ag6008 相同性解析による交差性が予測される生物種
Full Name MYB binding protein (P160) 1a
Calculated molecular weight 149 kDa
Observed molecular weight140 kDa
GenBank accession numberBC050546
Gene Symbol MYBBP1A
Gene ID (NCBI) 10514
RRIDAB_2918745
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BQG0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996].