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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated MYBPC3 Monoclonal antibody

MYBPC3 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C8F8

Cat no : CL594-67608

Synonyms

Cardiac MyBP C, CMH4, DKFZp779E1762, FHC, MYBP C, MYBPC3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite®594-conjugated MYBPC3 Monoclonal antibody (CL594-67608)

    WB analysis using CL594-67608

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-67608 (MYBPC3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inrat heart tissue, mouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67608 targets MYBPC3 in WB applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYBPC3 fusion protein Ag15848 相同性解析による交差性が予測される生物種
Full Name myosin binding protein C, cardiac
Calculated molecular weight 1274 aa, 141 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC151211
Gene symbol MYBPC3
Gene ID (NCBI) 4607
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4).

Protocols

Product Specific Protocols
WB protocol for CL594 MYBPC3 antibody CL594-67608Download protocol
Standard Protocols
Click here to view our Standard Protocols