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  • KD/KO Validated

c-MYC Monoclonal antibody

c-MYC Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 67447-1-Ig
Clone No.3D9C12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), IP, CoIP, ChIP, ELISA

MYC, MRTL, Class E basic helix-loop-helix protein 39, c Myc, bHLHe39

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inMDA-MB-231 cells, HT-29 cells, 4T1 cells, PC-3 cells, K-562 cells, HL-60 cells, Raji cells, HSC-T6 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, mouse brain tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inK-562 cells, NCCIT cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67447-1-Ig targets c-MYC in WB, IHC, IF, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen c-MYC fusion protein Ag17519 相同性解析による交差性が予測される生物種
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated molecular weight 49 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC000141
Gene Symbol MYC
Gene ID (NCBI) 4609
RRIDAB_2882681
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01106
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.

Protocols

Product Specific Protocols
WB protocol for c-MYC antibody 67447-1-IgDownload protocol
IHC protocol for c-MYC antibody 67447-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC

Gastroenterology

Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis

Authors - Xiao-Mei Yang
humanWB

Drug Resist Updat

Targeting ACYP1-mediated glycolysis reverses lenvatinib resistance and restricts hepatocellular carcinoma progression

Authors - Shuai Wang
  • KD Validated
human,mouseWB

Nat Commun

ID1 expressing macrophages support cancer cell stemness and limit CD8+ T cell infiltration in colorectal cancer

Authors - Shuang Shang
humanWB

Mol Cell

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Authors - Youming Zhu
human,mouseWB

Acta Pharm Sin B

Co-delivery of photosensitizer and diclofenac through sequentially responsive bilirubin nanocarriers for combating hypoxic tumors.

Authors - Yang Zhou
humanWB,IHC,IF

Theranostics

Hypoxia-induced TGFBI maintains glioma stem cells by stabilizing EphA2

Authors - Zirong Chen
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