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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

c-MYC Monoclonal antibody, PBS Only

c-MYC Monoclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D9C12

Cat no : 67447-1-PBS

Synonyms

bHLHe39, c Myc, c-MYC, MRTL, MYC, Myc proto oncogene protein, Proto oncogene c Myc, Transcription factor p64

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using c-MYC Monoclonal antibody (67447-1-Ig)

    WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67447-1-Ig (MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using c-MYC Monoclonal antibody (67447-1-Ig)

    WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67447-1-Ig (c-MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using c-MYC Monoclonal antibody (67447-1-Ig)

    WB analysis of 4T1 using 67447-1-Ig (same clone as 67447-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67447-1-Ig (c-MYC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using c-MYC Monoclonal antibody (67447-1-Ig)

    IHC staining of human colon cancer using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using c-MYC Monoclonal antibody (67447-1-Ig)

    IHC staining of human cervical cancer using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using c-MYC Monoclonal antibody (67447-1-Ig)

    IHC staining of human tonsillitis using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using c-MYC Monoclonal antibody (67447-1-Ig)

    IHC staining of mouse brain using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using c-MYC Monoclonal antibody (67447-1-Ig)

    IHC staining of mouse brain using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using c-MYC Monoclonal antibody (67447-1-Ig)

    IHC staining of human tonsillitis using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using c-MYC Monoclonal antibody (67447-1-Ig)

    IHC staining of human colon cancer using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using c-MYC Monoclonal antibody (67447-1-Ig)

    FC experiment of K-562 using 67447-1-Ig (same clone as 67447-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human c-MYC (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67447-1-PBS targets c-MYC in WB, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen c-MYC fusion protein Ag17519 相同性解析による交差性が予測される生物種
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated molecular weight 49 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC000141
Gene symbol MYC
Gene ID (NCBI) 4609
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.