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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using c-MYC Monoclonal antibody (67447-1-Ig)

    WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67447-1-Ig (MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using c-MYC Monoclonal antibody (67447-1-Ig)

    WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67447-1-Ig (c-MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using c-MYC Monoclonal antibody (67447-1-Ig)

    WB analysis of 4T1 using 67447-1-Ig (same clone as 67447-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67447-1-Ig (c-MYC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using c-MYC Monoclonal antibody (67447-1-Ig)

    IHC staining of human colon cancer using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using c-MYC Monoclonal antibody (67447-1-Ig)

    IHC staining of human cervical cancer using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using c-MYC Monoclonal antibody (67447-1-Ig)

    IHC staining of human tonsillitis using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using c-MYC Monoclonal antibody (67447-1-Ig)

    IHC staining of mouse brain using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using c-MYC Monoclonal antibody (67447-1-Ig)

    IHC staining of mouse brain using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using c-MYC Monoclonal antibody (67447-1-Ig)

    IHC staining of human tonsillitis using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using c-MYC Monoclonal antibody (67447-1-Ig)

    IHC staining of human colon cancer using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using c-MYC Monoclonal antibody (67447-1-Ig)

    FC experiment of HeLa using 67447-1-Ig (same clone as 67447-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug c-MYC Monoclonal antibody (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using c-MYC Monoclonal antibody (67447-1-Ig)

    FC experiment of K-562 using 67447-1-Ig (same clone as 67447-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human c-MYC (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of NCCIT cells using c-MYC Monoclonal antibody (67447-1-Ig)

    FC experiment of NCCIT using 67447-1-Ig (same clone as 67447-1-PBS)

    1x10^6 NCCIT cells were intracellularly stained with 0.25 ug c-MYC Monoclonal antibody (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

c-MYC Monoclonal antibody, PBS Only

c-MYC Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 67447-1-PBS
Clone No.3D9C12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

MYC, MRTL, Class E basic helix-loop-helix protein 39, c Myc, bHLHe39

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67447-1-PBS targets c-MYC in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen c-MYC fusion protein Ag17519 相同性解析による交差性が予測される生物種
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated molecular weight 49 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC000141
Gene Symbol MYC
Gene ID (NCBI) 4609
RRIDAB_2882681
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01106
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.