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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MYH1 Monoclonal antibody

MYH1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G10H9

Cat no : CL488-67299

Synonyms

MYH1, MYHa, MyHC 2x, MyHC 2X/D, MyHC IIx/d, MYHSA1, Myosin 1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using CoraLite® Plus 488-conjugated MYH1 Monoclonal anti (CL488-67299)

    IF Staining of mouse skeletal muscle using CL488-67299

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite® Plus 488 MYH1 antibody (CL488-67299, Clone: 1G10H9 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67299 targets MYH1 in IF-P applications and shows reactivity with Human, mouse, pig samples.

Tested Reactivity Human, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MYH1 fusion protein Ag17129 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 1, skeletal muscle, adult
Calculated molecular weight 1939 aa, 223 kDa
Observed molecular weight 220 kDa
GenBank accession numberBC114545
Gene symbol MYH1
Gene ID (NCBI) 4619
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MYH1 antibody CL488-67299Download protocol
Standard Protocols
Click here to view our Standard Protocols