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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated MYH10 Polyclonal ant (CL488-21403)

    IF Staining of HepG2 using CL488-21403

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MYH10 antibody (CL488-21403) at dilution of 1:200, CL594-Phalloidin (red).

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CoraLite® Plus 488-conjugated MYH10 Polyclonal antibody

MYH10 Polyclonal Antibody for IF/ICC
Cat No. CL488-21403

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

MYH10, Myosin 10, Myosin heavy chain 10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-21403 targets MYH10 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYH10 fusion protein Ag16113 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 10, non-muscle
Calculated molecular weight 1985 aa, 230 kDa
Observed molecular weight 229 kDa
GenBank accession numberBC150634
Gene Symbol MYH10
Gene ID (NCBI) 4628
RRIDAB_3672730
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35580
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MYH10 antibody CL488-21403Download protocol
Standard Protocols
Click here to view our Standard Protocols