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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated MYH10 Monoclonal antibody

MYH10 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2H8G7

Cat no : CL594-67243

Synonyms

MYH10, Myosin 10, Myosin heavy chain 10

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using CoraLite®594-conjugated MYH10 Monoclonal antibody (CL594-67243)

    IF Staining of mouse cerebellum using CL594-67243

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite®594-conjugated MYH10 antibody (CL594-67243, Clone: 2H8G7 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using CoraLite®594-conjugated MYH10 Monoclonal antibody (CL594-67243)

    IF Staining of mouse cerebellum using CL594-67243

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite®594-conjugated MYH10 antibody (CL594-67243, Clone: 2H8G7 ) at dilution of 1:100.

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Tested Applications

Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67243 targets MYH10 in IF-P applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MYH10 fusion protein Ag17069 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 10, non-muscle
Calculated molecular weight 1985 aa, 230 kDa
Observed molecular weight 200-230 kDa
GenBank accession numberBC150634
Gene symbol MYH10
Gene ID (NCBI) 4628
RRIDAB_2920102
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.

Protocols

Product Specific Protocols
IF protocol for CL594 MYH10 antibody CL594-67243Download protocol
Standard Protocols
Click here to view our Standard Protocols