SMMHC Monoclonal antibody
SMMHC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig, rabbit
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
5C6D3
Cat no : 60222-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | rabbit stomach tissue, human ileum tissue, rat rectum tissue, HeLa cells, rabbit large intestine, pig stomach, pig rectum, rat colon, mouse colon |
| Positive IHC detected in | human breast hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 7 publications below |
Product Information
60222-1-Ig targets SMMHC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SMMHC fusion protein Ag13397 相同性解析による交差性が予測される生物種 |
| Full Name | myosin, heavy chain 11, smooth muscle |
| Calculated molecular weight | 1938 aa, 224 kDa |
| Observed molecular weight | 220 kDa |
| GenBank accession number | BC101677 |
| Gene symbol | SMMHC/MYH11 |
| Gene ID (NCBI) | 4629 |
| RRID | AB_11182594 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SMMHC antibody 60222-1-Ig | Download protocol |
| IHC protocol for SMMHC antibody 60222-1-Ig | Download protocol |
| IF protocol for SMMHC antibody 60222-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Mol Basis Dis Methamphetamine induces thoracic aortic aneurysm/dissection through C/EBPβ. | ||
Atherosclerosis Nrf2 deficiency attenuates atherosclerosis by reducing LOX-1-mediated proliferation and migration of vascular smooth muscle cells. | ||
Nutr Metab Cardiovasc Dis Effects of carotid baroreceptor stimulation on aortic remodeling in obese rats. | ||
Int J Mol Sci Dietary Evodiamine Inhibits Atherosclerosis-Associated Changes in Vascular Smooth Muscle Cells | ||
Mol Cell Biochem HRD1 reduction promotes cholesterol-induced vascular smooth muscle cell phenotypic change via endoplasmic reticulum stress | ||
J Vasc Res Isolation and Cultivation of Vascular Smooth Muscle Cells from the Mouse Circle of Willis |
