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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMMHC Monoclonal antibody, PBS Only

SMMHC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5C6D3

Cat no : 60222-1-PBS

Synonyms

AAT4, DKFZp686D10126, DKFZp686D19237, FAA4, FLJ35232, KIAA0866, MYH11, Myosin 11, Myosin heavy chain 11, SMHC, SMMHC, SMM-HC, smooth muscle myosin heavy chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMMHC Monoclonal antibody (60222-1-Ig)

    WB analysis using 60222-1-Ig (same clone as 60222-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SMMHC Monoclonal antibody (60222-1-Ig)

    WB analysis using 60222-1-Ig (same clone as 60222-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human ileum tissue using SMMHC Monoclonal antibody (60222-1-Ig)

    WB analysis of human ileum using 60222-1-Ig (same clone as 60222-1-PBS)

    human ileum tissue were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SMMHC Monoclonal antibody (60222-1-Ig)

    WB analysis of HeLa using 60222-1-Ig (same clone as 60222-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using SMMHC Monoclonal antibody (60222-1-Ig)

    IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using SMMHC Monoclonal antibody (60222-1-Ig)

    IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using SMMHC Monoclonal antibody (60222-1-Ig)

    IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SMMHC Monoclonal antibody (60222-1-Ig)

    IF Staining of HeLa using 60222-1-Ig (same clone as 60222-1-PBS)

    Immunofluorescent analysis of HeLa cells using 60222-1-Ig (SMMHC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60222-1-PBS targets SMMHC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMMHC fusion protein Ag13397 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 11, smooth muscle
Calculated molecular weight 1938 aa, 224 kDa
Observed molecular weight 220 kDa
GenBank accession numberBC101677
Gene symbol SMMHC/MYH11
Gene ID (NCBI) 4629
RRIDAB_11182594
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.