- Featured Product
- KD/KO Validated
MYH14 Monoclonal antibody
MYH14 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse and More (1)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1B4C9
Cat no : 66825-1-Ig
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | COLO 320 cells, HT-29 cells |
| Positive IHC detected in | human colon tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IF | See 2 publications below |
Product Information
66825-1-Ig targets MYH14 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MYH14 fusion protein Ag28063 相同性解析による交差性が予測される生物種 |
| Full Name | myosin, heavy chain 14 |
| Calculated molecular weight | 228 kDa |
| Observed molecular weight | 200-230 kDa |
| GenBank accession number | NM_001077186 |
| Gene symbol | MYH14 |
| Gene ID (NCBI) | 79784 |
| RRID | AB_2882168 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYH14 antibody 66825-1-Ig | Download protocol |
| IHC protocol for MYH14 antibody 66825-1-Ig | Download protocol |
| IF protocol for MYH14 antibody 66825-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Sci Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation
| ||
J Cell Sci Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation. |
