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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using MYH14 Monoclonal antibody (66825-1-Ig)

    WB analysis of COLO 320 using 66825-1-Ig (same clone as 66825-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using MYH14 Monoclonal antibody (66825-1-Ig)

    WB analysis of HT-29 using 66825-1-Ig (same clone as 66825-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MYH14 Monoclonal antibody (66825-1-Ig)

    IHC staining of human colon using 66825-1-Ig (same clone as 66825-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MYH14 Monoclonal antibody (66825-1-Ig)

    IHC staining of human colon using 66825-1-Ig (same clone as 66825-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using MYH14 Monoclonal antibody (66825-1-Ig)

    IHC staining of mouse small intestine using 66825-1-Ig (same clone as 66825-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using MYH14 Monoclonal antibody (66825-1-Ig)

    IHC staining of mouse small intestine using 66825-1-Ig (same clone as 66825-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using MYH14 Monoclonal antibody (66825-1-Ig)

    IF Staining of mouse skeletal muscle using 66825-1-Ig (same clone as 66825-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 66825-1-Ig (MYH14 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

MYH14 Monoclonal antibody, PBS Only

MYH14 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66825-1-PBS
Clone No.1B4C9

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

myosin, MHC16, KIAA2034, FP17425, DFNA4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66825-1-PBS targets MYH14 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MYH14 fusion protein Ag28063 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 14
Calculated molecular weight 228 kDa
Observed molecular weight 200-230 kDa
GenBank accession numberNM_001077186
Gene Symbol MYH14
Gene ID (NCBI) 79784
RRIDAB_2882168
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z406
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4).