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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MYL3 Polyclonal antibody

MYL3 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10913

Synonyms

Ventricular myosin alkali light chain, Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using CoraLite® Plus 488-conjugated MYL3 Polyclonal anti (CL488-10913)

    IF Staining of C2C12 using CL488-10913

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CoraLite® Plus 488 MYL3 antibody (CL488-10913) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of C2C12 cells using CoraLite® Plus 488-conjugated MYL3 Polyclonal anti (CL488-10913)

    FC experiment of C2C12 using CL488-10913

    1x10^6 C2C12 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Myl3 Polyclonal Antibody (CL488-10913)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inC2C12 cells
Positive FC (Intra) detected inC2C12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10913 targets MYL3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYL3 fusion protein Ag1364 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 3, alkali; ventricular, skeletal, slow
Calculated molecular weight 22 kDa
Observed molecular weight 22-27 kDa
GenBank accession numberBC009790
Gene symbol MYL3
Gene ID (NCBI) 4634
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MYL3 antibody CL488-10913Download protocol
FC protocol for CL Plus 488 MYL3 antibody CL488-10913Download protocol
Standard Protocols
Click here to view our Standard Protocols