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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MYL3 Monoclonal antibody

MYL3 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E4B2

Cat no : CL488-66286

Synonyms

Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb, MLC1V

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using CoraLite® Plus 488-conjugated MYL3 Monoclonal anti (CL488-66286)

    IF Staining of mouse skeletal muscle using CL488-66286

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CL488-66286 (MYL3 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using CoraLite® Plus 488-conjugated MYL3 Monoclonal anti (CL488-66286)

    IF Staining of mouse skeletal muscle using CL488-66286

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CL488-66286 (MYL3 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using CoraLite® Plus 488-conjugated MYL3 Monoclonal anti (CL488-66286)

    IF Staining of C2C12 using CL488-66286

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CL488-66286 (MYL3 antibody) at dilution of 1:100.

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Tested Applications

Positive IF-P detected inmouse skeletal muscle tissue
Positive IF/ICC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66286 targets MYL3 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYL3 fusion protein Ag24535 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 3, alkali; ventricular, skeletal, slow
Calculated molecular weight 22 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC009790
Gene symbol MYL3
Gene ID (NCBI) 4634
RRIDAB_2883304
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MYL3 antibody CL488-66286Download protocol
Standard Protocols
Click here to view our Standard Protocols