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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated MYL7 Recombinant ant (CL750-81570)

    WB analysis using CL750-81570

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-81570 (MYL7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of C2C12 cells using CoraLite® Plus 750-conjugated MYL7 Recombinant ant (CL750-81570)

    FC experiment of C2C12 using CL750-81570

    1x10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated MYL7 Recombinant antibody (CL750-81570, Clone:2C5)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 750-conjugated MYL7 Recombinant antibody

MYL7 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-81570
Clone No.2C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Synonyms

MYLC2A, MYL7/MLC2a, MYL2A, MLC-2a, MLC2a

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size/Concentration:  100 μL, 1000 ug/ml
100 μL, 1000 ug/ml

¥85,000/ 100 μL

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat heart tissue, mouse heart tissue
Positive FC (Intra) detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-81570 targets MYL7 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYL7 fusion protein Ag11058 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 7, regulatory
Calculated molecular weight 175 aa, 19 kDa
Observed molecular weight19 kDa
GenBank accession numberBC027915
Gene Symbol MYL7
Gene ID (NCBI) 58498
RRIDAB_3673731
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01449
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 MYL7 antibody CL750-81570Download protocol
FC protocol for CL Plus 750 MYL7 antibody CL750-81570Download protocol
Standard Protocols
Click here to view our Standard Protocols