MYLK Polyclonal antibody

Name: MYLK Polyclonal antibody
Brand: Proteintech
SKU: 21642-1-AP

MYLK Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21642-1-AP

Synonyms

MLCK108, MLCK1, MLCK, KRP, Kinase-related protein

Validation Data Gallery

Filter:

AllWBIHCIF-PIF/ICC

WB analysis of mouse uterus using 21642-1-AP
mouse uterus tissue were subjected to SDS PAGE followed by western blot with 21642-1-AP (MYLK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse large intestine using 21642-1-AP
mouse large intestine tissue were subjected to SDS PAGE followed by western blot with 21642-1-AP (MYLK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse uterus using 21642-1-AP
mouse uterus tissue were subjected to SDS PAGE followed by western blot with 21642-1-AP (MYLK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 21642-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21642-1-AP (MYLK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 21642-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21642-1-AP (MYLK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse ovary using 21642-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using MYLK antibody (21642-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 21642-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYLK antibody (21642-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Cat No. 21642-1-AP

Size/Concentration

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse uterus tissue, mouse large intestine tissue
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse ovary tissue
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 19 publications below
IHC	See 3 publications below
IF	See 5 publications below
CoIP	See 1 publications below

Product Information

21642-1-AP targets MYLK in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MYLK fusion protein Ag16306 相同性解析による交差性が予測される生物種
Full Name	myosin light chain kinase
Calculated molecular weight	1914 aa, 211 kDa
Observed molecular weight	135 kDa
GenBank accession number	BC113456
Gene symbol	MYLK
Gene ID (NCBI)	4638
RRID	AB_10734590
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MYLK(Myosin light chain kinase) is also named as MLCK, MLCK1, MYLK1 and belongs to the protein kinase superfamily. It is a key enzyme in muscle contraction. Its activation is sufficient to enhance paracellular permeability and is required for tight junction barrier regulation in response to Na+-nutrient cotransport, inflammatory cytokines, or pathogenic bacteria(PMID:20404178). The expression of smooth muscle MYLK is tissue-specific. Smooth muscle MYLK generates three different proteins, long MYLK, short MYLK, and telokin. The 130-kDa smooth muscle, or short, MYLK (130 kDa) is expressed in smooth muscles. It has 8 isoforms produced by alternative splicing and alternative initiation and our anti-MYLK antibody can recognize these isforms.

Protocols

Product Specific Protocols
WB protocol for MYLK antibody 21642-1-AP	Download protocol
IHC protocol for MYLK antibody 21642-1-AP	Download protocol
IF protocol for MYLK antibody 21642-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Research (Wash D C) The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling Authors - Xi Chen View Article
human	WB	Mucosal Immunol Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment. Authors - R Sumagin View Article
mouse	WB,IHC	Int J Biol Sci Aryl Hydrocarbon Receptor Activation Modulates Intestinal Epithelial Barrier Function by Maintaining Tight Junction Integrity. Authors - Min Yu View Article
rat	IF	FEBS J Dihydroartemisinin counteracts fibrotic portal hypertension via farnesoid X receptor-dependent inhibition of hepatic stellate cell contraction. Authors - Wenxuan Xu View Article
human	WB	Front Med (Lausanne) Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer. Authors - Fang He View Article
mouse	WB	Metallomics Lanthanum chloride causes blood-brain barrier disruption through intracellular calcium-mediated RhoA/Rho kinase signaling and myosin light chain kinase. Authors - Jie Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYLK Polyclonal antibody

MYLK Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Validation Data Gallery

WB analysis of mouse uterus using 21642-1-AP

WB analysis of mouse large intestine using 21642-1-AP

WB analysis of mouse uterus using 21642-1-AP

IHC staining of human colon using 21642-1-AP

IHC staining of human colon using 21642-1-AP

IF Staining of mouse ovary using 21642-1-AP

IF Staining of HepG2 using 21642-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling

Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment.

Aryl Hydrocarbon Receptor Activation Modulates Intestinal Epithelial Barrier Function by Maintaining Tight Junction Integrity.

Dihydroartemisinin counteracts fibrotic portal hypertension via farnesoid X receptor-dependent inhibition of hepatic stellate cell contraction.

Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.

Lanthanum chloride causes blood-brain barrier disruption through intracellular calcium-mediated RhoA/Rho kinase signaling and myosin light chain kinase.