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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human rectal cancer tissue using CoraLite® Plus 488-conjugated MYLK Polyclonal anti (CL488-21642)

    IF Staining of human rectal cancer using CL488-21642

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human rectal cancer tissue using CoraLite® Plus 488 MYLK antibody (CL488-21642) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CoraLite® Plus 488-conjugated MYLK Polyclonal antibody

MYLK Polyclonal Antibody for IF-P
Cat No. CL488-21642

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman rectal cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-21642 targets MYLK in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYLK fusion protein Ag16306 相同性解析による交差性が予測される生物種
Full Name myosin light chain kinase
Calculated molecular weight 1914 aa, 211 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC113456
Gene Symbol MYLK
Gene ID (NCBI) 4638
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15746
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYLK(Myosin light chain kinase) is also named as MLCK, MLCK1, MYLK1 and belongs to the protein kinase superfamily. It is a key enzyme in muscle contraction. Its activation is sufficient to enhance paracellular permeability and is required for tight junction barrier regulation in response to Na+-nutrient cotransport, inflammatory cytokines, or pathogenic bacteria(PMID:20404178). The expression of smooth muscle MYLK is tissue-specific. Smooth muscle MYLK generates three different proteins, long MYLK, short MYLK, and telokin. The 130-kDa smooth muscle, or short, MYLK (130 kDa) is expressed in smooth muscles. It has 8 isoforms produced by alternative splicing and alternative initiation and our anti-MYLK antibody can recognize these isforms.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MYLK antibody CL488-21642Download protocol
Standard Protocols
Click here to view our Standard Protocols