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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYLK3 Polyclonal antibody (21527-1-AP)

    WB analysis using 21527-1-AP (same clone as 21527-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21527-1-AP (MYLK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using MYLK3 Polyclonal antibody (21527-1-AP)

    WB analysis of human heart using 21527-1-AP (same clone as 21527-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 21527-1-AP (MYLK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MYLK3 Polyclonal antibody (21527-1-AP)

    IHC staining of human colon using 21527-1-AP (same clone as 21527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MYLK3 Polyclonal antibody (21527-1-AP)

    IHC staining of human colon using 21527-1-AP (same clone as 21527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MYLK3 Polyclonal antibody (21527-1-AP)

    IHC staining of human heart using 21527-1-AP (same clone as 21527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MYLK3 Polyclonal antibody (21527-1-AP)

    IHC staining of human heart using 21527-1-AP (same clone as 21527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYLK3 Polyclonal antibody (21527-1-AP)

    IF Staining of HepG2 using 21527-1-AP (same clone as 21527-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYLK3 antibody (21527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYLK3 Polyclonal antibody (21527-1-AP)

    IF Staining of HepG2 using 21527-1-AP (same clone as 21527-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

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MYLK3 Polyclonal antibody, PBS Only

MYLK3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21527-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21527-1-PBS targets MYLK3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYLK3 fusion protein Ag16137 相同性解析による交差性が予測される生物種
Full Name myosin light chain kinase 3
Calculated molecular weight 819 aa, 88 kDa
Observed molecular weight 85-100 kDa
GenBank accession numberBC109097
Gene Symbol MYLK3
Gene ID (NCBI) 91807
RRIDAB_10734432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ32MK0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cardiac myosin light chain kinase (cMLCK) encoded by the myosin light chain kinase 3 (MYLK3) phosphorylates cardiac myosin light chain 2 (MLC2, encoded by MYL2) and facilitates actin-myosin interactions that enhance contractile force in the animal heart (PMID: 29235529).