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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYO1G Recombinant antibody (83556-5-RR)

    WB analysis using 83556-5-RR (same clone as 83556-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83556-5-RR (MYO1G antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using MYO1G Recombinant antibody (83556-5-RR)

    IF Staining of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (83556-5-RR, Clone: 240503E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using MYO1G Recombinant antibody (83556-5-RR)

    IF Staining of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (83556-5-RR, Clone: 240503E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using MYO1G Recombinant antibody (83556-5-RR)

    FC experiment of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MYO1G Recombinant antibody (83556-5-RR, Clone:240503E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83556-5-RR

    Affinity and Kinetic Characterization of 83556-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83556-5-RR against Human MYO1G were performed. The affinity constant is 1.03 nM.

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MYO1G Recombinant antibody, PBS Only

MYO1G Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83556-5-PBS
Clone No.240503E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Unconventional myosin-Ig, myosin IG, Minor histocompatibility antigen HA-2, HA 2, 240503E1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83556-5-PBS targets MYO1G in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYO1G fusion protein Ag22698 相同性解析による交差性が予測される生物種
Full Name myosin IG
Calculated molecular weight 1018 aa, 116 kDa
Observed molecular weight110-120 kDa
GenBank accession numberBC113544
Gene Symbol MYO1G
Gene ID (NCBI) 64005
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDB0I1T2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MYO1G is a plasma membrane-associated class I myosin that is abundant in T and B lymphocytes and mast cells and expressed specifically in the haematopoietic system. Class I myosins have been implicated in various cellular processes relying on actin-dependent membrane dynamics such as endocytosis, secretion, adhesion, motility and regulation of mechanosensitive channels (PMID: 19968988). MYO1G localizes to the plasma membrane linked to PIP2 and PIP3, is particularly enriched at cell-surface microvilli, and associates in an ATP-releasable manner to the actin cytoskeleton (PMID: 24310084). MYO1G can be detected as about 110-120 kDa.