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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYO1G Recombinant antibody (83556-5-RR)

    WB analysis using 83556-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83556-5-RR (MYO1G antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using MYO1G Recombinant antibody (83556-5-RR)

    IF Staining of Jurkat using 83556-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (83556-5-RR, Clone: 240503E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using MYO1G Recombinant antibody (83556-5-RR)

    IF Staining of Jurkat using 83556-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (83556-5-RR, Clone: 240503E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of Jurkat cells using MYO1G Recombinant antibody (83556-5-RR)

    FC experiment of Jurkat using 83556-5-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MYO1G Recombinant antibody (83556-5-RR, Clone:240503E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83556-5-RR

    Affinity and Kinetic Characterization of 83556-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83556-5-RR against Human MYO1G were performed. The affinity constant is 1.03 nM.

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MYO1G Recombinant antibody

MYO1G Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83556-5-RR
Clone No.240503E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Unconventional myosin-Ig, myosin IG, Minor histocompatibility antigen HA-2, HA 2, 240503E1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, Ramos cells
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83556-5-RR targets MYO1G in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYO1G fusion protein Ag22698 相同性解析による交差性が予測される生物種
Full Name myosin IG
Calculated molecular weight 1018 aa, 116 kDa
Observed molecular weight110-120 kDa
GenBank accession numberBC113544
Gene Symbol MYO1G
Gene ID (NCBI) 64005
RRIDAB_3671173
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDB0I1T2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYO1G is a plasma membrane-associated class I myosin that is abundant in T and B lymphocytes and mast cells and expressed specifically in the haematopoietic system. Class I myosins have been implicated in various cellular processes relying on actin-dependent membrane dynamics such as endocytosis, secretion, adhesion, motility and regulation of mechanosensitive channels (PMID: 19968988). MYO1G localizes to the plasma membrane linked to PIP2 and PIP3, is particularly enriched at cell-surface microvilli, and associates in an ATP-releasable manner to the actin cytoskeleton (PMID: 24310084). MYO1G can be detected as about 110-120 kDa.

Protocols

Product Specific Protocols
WB protocol for MYO1G antibody 83556-5-RRDownload protocol
IF protocol for MYO1G antibody 83556-5-RRDownload protocol
FC protocol for MYO1G antibody 83556-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols