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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYO6 Recombinant antibody

MYO6 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241251G11

Cat no : 84085-3-RR

Synonyms

Unconventional myosin 6, myosin VI, KIAA0389, DFNB37, DFNA22

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYO6 Recombinant antibody (84085-3-RR)

    WB analysis using 84085-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84085-3-RR (MYO6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using MYO6 Recombinant antibody (84085-3-RR)

    WB analysis of LNCaP using 84085-3-RR

    LNCaP cells were subjected to SDS PAGE followed by western blot with 84085-3-RR (MYO6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inMCF-7 cells, LNCaP cells, HEK-293T cells, DU 145 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84085-3-RR is MYO6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYO6 fusion protein Ag24906 相同性解析による交差性が予測される生物種
Full Name myosin VI
Calculated molecular weight 1285 aa, 149 kDa
Observed molecular weight 145-150 kDa
GenBank accession numberBC146764
Gene symbol MYO6
Gene ID (NCBI) 4646
RRIDAB_3671653
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYO6, an actin-based motor protein, is the only myosin known to move toward the minus end of actin filaments. MYO6 is highly expressed in the inner and outer hair cells of the ear, retina, and polarized epithelial cells such as kidney proximal tubule cells and intestinal enterocytes. And it participates in a wide range of biological processes within cells, including clathrin-mediated endocytosis, vesicular membrane traffic, polarized secretion, and autophagy (PMID: 23620821; PMID: 28591580). Previous studies showed that MYO6 is upregulated in various types of cancer, and it has been widely reported to contribute to tumor cell migration and metastasis. Some articles indicate that MYO6 is associated with prostate cancer, lung cancer, human colorectal cancer and gastric cancer (PMID: 29022908).

Protocols

Product Specific Protocols
WB protocol for MYO6 antibody 84085-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols