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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYOM3 Recombinant antibody (83852-3-RR)

    WB analysis using 83852-3-RR (same clone as 83852-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83852-3-RR (MYOM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83852-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYOM3 Recombinant antibody (83852-3-RR)

    IHC staining of mouse skeletal muscle using 83852-3-RR (same clone as 83852-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83852-3-RR (MYOM3 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83852-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using MYOM3 Recombinant antibody (83852-3-RR)

    IF Staining of mouse skeletal muscle using 83852-3-RR (same clone as 83852-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYOM3 antibody (83852-3-RR, Clone: 240981F9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83852-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83852-3-RR

    Affinity and Kinetic Characterization of 83852-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83852-3-RR against Human MYOM3 were performed. The affinity constant is 0.106 nM.

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MYOM3 Recombinant antibody, PBS Only

MYOM3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83852-3-PBS
Clone No.240981F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Myomesin-3, Myomesin family member 3, Myomesin 3, 240981F9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83852-3-PBS targets MYOM3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYOM3 fusion protein Ag11849 相同性解析による交差性が予測される生物種
Full Name myomesin family, member 3
Calculated molecular weight 162 kDa, 136 kDa
Observed molecular weight 162 kDa
GenBank accession numberBC067101
Gene Symbol MYOM3
Gene ID (NCBI) 127294
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ5VTT5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MYOM3 (myomesin 3) is a structural component of the M-band in striated muscle and is involved in sarcomere stability and resistance during intense or sustained stretching. MYOM3 can be detected mainly in intermediate-speed fibers of skeletal muscle. Recently high levels of MYOM3 fragments were detected in sera from patients with muscular dystrophy, including Duchenne muscular dystrophy (DMD). MYOM3 fragments may be used as serum biomarkers for DMD and other neuromuscular disorders.