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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYOM3 Recombinant antibody

MYOM3 Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

240981F9

Cat no : 83852-3-RR

Synonyms

Myomesin-3, Myomesin family member 3, Myomesin 3, 240981F9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYOM3 Recombinant antibody (83852-3-RR)

    WB analysis using 83852-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83852-3-RR (MYOM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYOM3 Recombinant antibody (83852-3-RR)

    IHC staining of mouse skeletal muscle using 83852-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83852-3-RR (MYOM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using MYOM3 Recombinant antibody (83852-3-RR)

    IF Staining of mouse skeletal muscle using 83852-3-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYOM3 antibody (83852-3-RR, Clone: 240981F9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83852-3-RR

    Affinity and Kinetic Characterization of 83852-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83852-3-RR against Human MYOM3 were performed. The affinity constant is 0.106 nM.

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83852-3-RR is MYOM3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYOM3 fusion protein Ag11849 相同性解析による交差性が予測される生物種
Full Name myomesin family, member 3
Calculated molecular weight 162 kDa, 136 kDa
Observed molecular weight 162 kDa
GenBank accession numberBC067101
Gene symbol MYOM3
Gene ID (NCBI) 127294
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYOM3 (myomesin 3) is a structural component of the M-band in striated muscle and is involved in sarcomere stability and resistance during intense or sustained stretching. MYOM3 can be detected mainly in intermediate-speed fibers of skeletal muscle. Recently high levels of MYOM3 fragments were detected in sera from patients with muscular dystrophy, including Duchenne muscular dystrophy (DMD). MYOM3 fragments may be used as serum biomarkers for DMD and other neuromuscular disorders.

Protocols

Product Specific Protocols
WB protocol for MYOM3 antibody 83852-3-RRDownload protocol
IHC protocol for MYOM3 antibody 83852-3-RRDownload protocol
IF protocol for MYOM3 antibody 83852-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols