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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MCP-1/CCL2 Monoclonal antibody

MCP-1/CCL2 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B9F7

Cat no : CL488-66272

Synonyms

Ccl2, Mcp1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CoraLite® Plus 488-conjugated Mcp1 Monoclonal anti (CL488-66272)

    IF Staining of mouse liver using CL488-66272

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 Mcp1 antibody (CL488-66272, Clone: 1B9F7 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66272 targets MCP-1/CCL2 in IF-P applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MCP-1/CCL2 fusion protein Ag24085 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 2
Observed molecular weight 26 kDa
GenBank accession numberNM-011333
Gene symbol MCP-1/CCL2
Gene ID (NCBI) 20296
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MCP-1/CCL2 antibody CL488-66272Download protocol
Standard Protocols
Click here to view our Standard Protocols