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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse Mlkl Polyclonal antibody, PBS Only

Mlkl Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30145-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Mlkl Polyclonal antibody (30145-1-AP)

    WB analysis using 30145-1-AP (same clone as 30145-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30145-1-AP (Mlkl antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30145-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Mlkl Polyclonal antibody (30145-1-AP)

    WB analysis using 30145-1-AP (same clone as 30145-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30145-1-AP (Mlkl antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30145-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Mlkl Polyclonal antibody (30145-1-AP)

    IF Staining of NIH/3T3 using 30145-1-AP (same clone as 30145-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Mlkl antibody (30145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30145-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Mlkl Polyclonal antibody (30145-1-AP)

    IF Staining of HepG2 using 30145-1-AP (same clone as 30145-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Mlkl antibody (30145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30145-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30145-1-PBS targets Mlkl in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Mlkl fusion protein Ag32514 相同性解析による交差性が予測される生物種
Full Name mixed lineage kinase domain-like
Calculated molecular weight54kd
Observed molecular weight 54 kDa
GenBank accession numberNM_001310613
Gene symbol Mlkl
Gene ID (NCBI) 74568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Mixed lineage kinase domain-like pseudokinase (Mlkl) belongs to the protein kinase superfamily and has two MWs of 54 and 30 kDa. Mlkl plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in the necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.