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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mono/Di-Methyl-Histone H3 (Lys9) Recombinant antibody, PBS Only

Mono/Di-Methyl-Histone H3 (Lys9) Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E12

Cat no : 80219-1-PBS

Synonyms

HIST1H3A, 1E12, Histone-H3, HistoneH3, Histone H3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Mono/Di-Methyl-Histone H3 (Lys9) Recombinant antib (80219-1-RR)

    WB analysis using 80219-1-RR (same clone as 80219-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80219-1-RR (Mono/Di-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80219-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Mono/Di-Methyl-Histone H3 (Lys9) Recombinant antib (80219-1-RR)

    IF Staining of HeLa using 80219-1-RR (same clone as 80219-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono/Di-Methyl-Histone H3 (Lys9) antibody (80219-1-RR, Clone: 1E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80219-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Mono/Di-Methyl-Histone H3 (Lys9) Recombinant antib (80219-1-RR)

    IF Staining of HeLa using 80219-1-RR (same clone as 80219-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono/Di-Methyl-Histone H3 (Lys9) antibody (80219-1-RR, Clone: 1E12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80219-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80219-1-PBS targets Mono/Di-Methyl-Histone H3 (Lys9) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15-18 kDa
GenBank accession numberBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression. This antibody is specific to monomethyl or dimethyl-Histone H3 while it does not recognize trimethyl-Histone H3 (Lys9). It is also named as H3K9me1/2.