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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mono/Di-Methyl-Histone H3 (Lys9) Recombinant antibody

Mono/Di-Methyl-Histone H3 (Lys9) Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E12

Cat no : 80219-1-RR

Synonyms

HIST1H3A, 1E12, Histone-H3, HistoneH3, Histone H3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Mono/Di-Methyl-Histone H3 (Lys9) Recombinant antib (80219-1-RR)

    WB analysis using 80219-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80219-1-RR (Mono/Di-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Mono/Di-Methyl-Histone H3 (Lys9) Recombinant antib (80219-1-RR)

    IF Staining of HeLa using 80219-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono/Di-Methyl-Histone H3 (Lys9) antibody (80219-1-RR, Clone: 1E12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Mono/Di-Methyl-Histone H3 (Lys9) Recombinant antib (80219-1-RR)

    IF Staining of HeLa using 80219-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono/Di-Methyl-Histone H3 (Lys9) antibody (80219-1-RR, Clone: 1E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inTHP-1 cells, K-562 cells, Jurkat cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, C2C12 cells, HSC-T6 cells, PC-12 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80219-1-RR targets Mono/Di-Methyl-Histone H3 (Lys9) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15-18 kDa
GenBank accession numberBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_2918878
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression. This antibody is specific to monomethyl or dimethyl-Histone H3 while it does not recognize trimethyl-Histone H3 (Lys9). It is also named as H3K9me1/2.

Protocols

Product Specific Protocols
WB protocol for Mono/Di-Methyl-Histone H3 (Lys9) antibody 80219-1-RRDownload protocol
IF protocol for Mono/Di-Methyl-Histone H3 (Lys9) antibody 80219-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols