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Mouse IgG1 isotype control Monoclonal antibody

Mouse IgG1 isotype control Monoclonal Antibody for WB, FC, ELISA
Cat No. 66360-1-Ig
Clone No.1F8D3

Host / Isotype

Mouse / IgG1

Reactivity

megathura crenulata and More (1)

Applications

WB, IHC, IF, FC , IP, CoIP, RIP, Neutralization, ELISA

Mouse IgG1, Kappa light chain

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected in
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66360-1-Ig targets Mouse IgG1 isotype control in WB, IHC, IF, FC , IP, CoIP, RIP, Neutralization, ELISA applications and shows reactivity with megathura crenulata samples.

Tested Reactivity megathura crenulata
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Mouse IgG1 isotype control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2827991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Protocols

Product Specific Protocols
WB protocol for Mouse IgG1 isotype control antibody 66360-1-IgDownload protocol
FC protocol for Mouse IgG1 isotype control antibody 66360-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
FC

Nat Commun

EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses

Authors - Qiu Pan
CoIP

FASEB J

Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection

Authors - Chujie Ding
FC

J Immunol

Phagocytic Plasma Cells in Teleost Fish Provide Insights into the Origin and Evolution of B Cells in Vertebrates

Authors - Xue-Qing Han
IP

Exp Cell Res

CBL aggravates Ang II-induced cardiac hypertrophy via the VHL/HIF-1α pathway.

Authors - Yuting Yang
IHC

Am J Rhinol Allergy

Co-Expression and Localization of Angiotensin-Converting Enzyme-2 (ACE2) and the Transmembrane Serine Protease 2 (TMPRSS2) in Paranasal Ciliated Epithelium of Patients with Chronic Rhinosinusitis.

Authors - Tomohiro Kawasumi

bioRxiv

Immunojanus Particles for low-volume and isolation-free unlabeled characterization of small Extracellular Vesicle in biofluids: Characterization of disease type by surface marker profiling

Authors - Sonu Kumar
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