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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Mouse IgG1 isotype control Monoclonal antibody (66360-1-Ig)

    WB analysis using 66360-1-Ig (same clone as 66360-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66360-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Mouse IgG1 isotype control Monoclonal antibody (66360-1-Ig)

    WB analysis using 66360-1-Ig (same clone as 66360-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66360-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Mouse IgG1 isotype control Monoclonal antibody (66360-1-Ig)

    FC experiment of human PBMCs using 66360-1-Ig (same clone as 66360-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.5 ug Mouse IgG1 isotype control Monoclonal antibody (66360-1-Ig, Clone: 1F8D3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.5 ug Anti-Human CD3 (65151-1-Ig) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (green), or unstained (blue). Cells were not fixed. This data was developed using the same antibody clone with 66360-1-PBS in a different storage buffer formulation.

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Mouse IgG1 isotype control Monoclonal antibody, PBS Only

Mouse IgG1 isotype control Monoclonal Antibody for WB, ELISA
Cat No. 66360-1-PBS
Clone No.1F8D3

Host / Isotype

Mouse / IgG1

Reactivity

megathura crenulata

Applications

WB, ELISA

Synonyms

Mouse IgG1, Kappa light chain

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66360-1-PBS targets Mouse IgG1 isotype control in WB, ELISA applications and shows reactivity with megathura crenulata samples.

Tested Reactivity megathura crenulata
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Mouse IgG1 isotype control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2827991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).