ChromoTek Myc-Trap® Agarose, kit
Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.
Specificity
Myc-Tag
Applications
IP, CoIP, ChIP, RIP
Conjugate
Agarose
Type
Nanobody
Cat no : ytak
Synonyms
Validation Data Gallery
Product Information
Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.
| Description | The Myc-Trap Agarose Kit contains Myc-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of Myc-tagged proteins and their interacting factors.
• Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains on the SDS-PAGE • Stable under harsh washing conditions • Elution with peptide |
| Applications | IP, CoIP, ChIP, RIP |
| Specificity/Target | Myc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound. |
| Binding capacity | 17.5 μg of recombinant Myc-tagged protein (~42 kDa) per 25 μL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | 2x Myc peptide. SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | 1x Myc-tag: Dissociation constant KD of 500 nM 2x Myc-tag: Dissociation constant KD of 0.5 nM |
| Compatibility with mass spectrometry | The Myc-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631369 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| Myc-Trap® Agarose | 20 reactions (500 µL) |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |
Documentation
| SDS |
|---|
| SDS Myc-Trap Agarose Kit (PDF) |
| Protocol |
|---|
| Protocol Myc-Trap Agarose Kit (PDF) |
| Whitepapers |
|---|
| Tips & tricks for immunoprecipitation using the Myc-Trap (PDF) |
| Myc-Trap versus conventional anti-Myc antibodies (PDF) |
| Troubleshooting |
|---|
| Troubleshooting guide immunoprecipitation (PDF) |
| Brochure |
|---|
| Myc-Trap and anti-Myc antibody flyer (PDF) |
Publications
| Application | Title |
|---|---|
Proc Natl Acad Sci U S A Molecular determinants of homo- and heteromeric interactions of Junctophilin-1 at triads in adult skeletal muscle fibers. | |
Int J Mol Sci Yil102c-A is a Functional Homologue of the DPMII Subunit of Dolichyl Phosphate Mannose Synthase in Saccharomyces cerevisiae. | |
J Pharmacol Exp Ther Inhibiting Sialidase-Induced TGF-β1 Activation Attenuates Pulmonary Fibrosis in Mice. | |
PLoS One The interplay of Rac1 activity, ubiquitination and GDI binding and its consequences for endothelial cell spreading. |