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Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    WB analysis of human skeletal muscle using 66205-1-Ig (same clone as 66205-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    WB analysis of human heart using 66205-1-Ig (same clone as 66205-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit heart tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    WB analysis of rabbit heart using 66205-1-Ig (same clone as 66205-1-PBS)

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    WB analysis of pig heart using 66205-1-Ig (same clone as 66205-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    WB analysis of rat heart using 66205-1-Ig (same clone as 66205-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    WB analysis of mouse heart using 66205-1-Ig (same clone as 66205-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    IHC staining of human heart using 66205-1-Ig (same clone as 66205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66205-1-Ig (Myoglobin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    IHC staining of rat heart using 66205-1-Ig (same clone as 66205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse tongue tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    IHC staining of mouse tongue using 66205-1-Ig (same clone as 66205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of C2C12 cells using Myoglobin Monoclonal antibody (66205-1-Ig)

    FC experiment of C2C12 using 66205-1-Ig (same clone as 66205-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Myoglobin (66205-1-Ig, Clone:1A4F7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

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Myoglobin Monoclonal antibody, PBS Only

Myoglobin Monoclonal Antibody for Indirect ELISA, IHC, WB
Cat No. 66205-1-PBS
Clone No.1A4F7

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, pig, rat, mouse, human

Applications

Indirect ELISA, IHC, WB

Synonyms

MB, Pseudoperoxidase MB, Nitrite reductase MB, EC:1.7.-.-, EC:1.11.1.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66205-1-PBS targets Myoglobin in Indirect ELISA, IHC, WB applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

Tested Reactivity rabbit, pig, rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Myoglobin fusion protein Ag8979 相同性解析による交差性が予測される生物種
Full Name myoglobin
Calculated molecular weight 154 aa, 17 kDa
Observed molecular weight 17-18 kDa
GenBank accession numberBC014547
Gene Symbol Myoglobin
Gene ID (NCBI) 4151
RRIDAB_2881596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02144
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates.