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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Myoglobin Monoclonal antibody

Myoglobin Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A4F7

Cat no : 66205-1-Ig

Synonyms

MB, Pseudoperoxidase MB, Nitrite reductase MB, EC:1.7.-.-, EC:1.11.1.-

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    WB analysis of human skeletal muscle using 66205-1-Ig

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    WB analysis of human heart using 66205-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig heart tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    WB analysis of pig heart using 66205-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    WB analysis of rat heart using 66205-1-Ig

    rat heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    WB analysis of mouse heart using 66205-1-Ig

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit heart tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    WB analysis of rabbit heart using 66205-1-Ig

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    IHC staining of human heart using 66205-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66205-1-Ig (Myoglobin antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat heart tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    IHC staining of rat heart using 66205-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse tongue tissue using Myoglobin Monoclonal antibody (66205-1-Ig)

    IHC staining of mouse tongue using 66205-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of C2C12 cells using Myoglobin Monoclonal antibody (66205-1-Ig)

    FC experiment of C2C12 using 66205-1-Ig

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Myoglobin (66205-1-Ig, Clone:1A4F7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inhuman skeletal muscle tissue, pig heart tissue, rat heart tissue, mouse heart tissue, rabbit heart tissue, human heart tissue
Positive IHC detected inhuman heart tissue, mouse tongue tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:80000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66205-1-Ig targets Myoglobin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivitypig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Myoglobin fusion protein Ag8979 相同性解析による交差性が予測される生物種
Full Name myoglobin
Calculated molecular weight 154 aa, 17 kDa
Observed molecular weight 17-18 kDa
GenBank accession numberBC014547
Gene symbol Myoglobin
Gene ID (NCBI) 4151
RRIDAB_2881596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates.

Protocols

Product Specific Protocols
WB protocol for Myoglobin antibody 66205-1-IgDownload protocol
IHC protocol for Myoglobin antibody 66205-1-IgDownload protocol
FC protocol for Myoglobin antibody 66205-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Stress Biol

Time-restricted feeding relieves high temperature-induced impairment on meat quality by activating the Nrf2/HO-1 pathway, modification of muscle fiber composition, and enriching the polyunsaturated fatty acids in pigs

Authors - Zhaojian Li
View Article