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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Myoglobin Monoclonal antibody

Myoglobin Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A4F7

Cat no : CL488-66205

Synonyms

MB, Pseudoperoxidase MB, Nitrite reductase MB, EC:1.7.-.-, EC:1.11.1.-

Validation Data Gallery

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  • Flow cytometry (FC) experiment of C2C12 cells using CoraLite® Plus 488-conjugated Myoglobin Monoclonal (CL488-66205)

    FC experiment of C2C12 using CL488-66205

    1X10^6 C2C12 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Myoglobin (CL488-66205, Clone:1A4F7) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inC2C12 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66205 targets Myoglobin in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Myoglobin fusion protein Ag8979 相同性解析による交差性が予測される生物種
Full Name myoglobin
Calculated molecular weight 154 aa, 17 kDa
Observed molecular weight 17-18 kDa
GenBank accession numberBC014547
Gene symbol Myoglobin
Gene ID (NCBI) 4151
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Myoglobin antibody CL488-66205Download protocol
Standard Protocols
Click here to view our Standard Protocols