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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Myosin Light Chain 2 Monoclonal antibody

Myosin Light Chain 2 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5A1F1

Cat no : CL594-60229

Synonyms

MLC 2v, MYL2, MLC-2s/v, MLC-2, MLC2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated Myosin Light Chain 2 Monoc (CL594-60229)

    IF Staining of mouse heart using CL594-60229

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite®594 Myosin Light Chain 2 antibody (CL594-60229, Clone: 5A1F1 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using CoraLite®594-conjugated Myosin Light Chain 2 Monoc (CL594-60229)

    IF Staining of C2C12 using CL594-60229

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CL594-60229 (Myosin Light Chain 2 antibody) at dilution of 1:50. .

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Tested Applications

Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60229 targets Myosin Light Chain 2 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Myosin Light Chain 2 fusion protein Ag1356 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 2, regulatory, cardiac, slow
Calculated molecular weight 19 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC015821
Gene symbol Myosin Light Chain 2
Gene ID (NCBI) 4633
RRIDAB_2883447
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

Protocols

Product Specific Protocols
IF protocol for CL594 Myosin Light Chain 2 antibody CL594-60229Download protocol
Standard Protocols
Click here to view our Standard Protocols