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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated N-PAC Monoclonal antibody

N-PAC Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A5E1

Cat no : CL488-67340

Synonyms

GLYR1, HIBDL, Glyoxylate reductase 1 homolog, Cytokine-like nuclear factor N-PAC, 3-hydroxyisobutyrate dehydrogenase-like protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated N-PAC Monoclonal ant (CL488-67340)

    IF Staining of HeLa using CL488-67340

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 N-PAC antibody (CL488-67340, Clone: 1A5E1 ) at dilution of 1:600.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated N-PAC Monoclonal ant (CL488-67340)

    FC experiment of HeLa using CL488-67340

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human N-PAC (CL488-67340, Clone:1A5E1) and at dilution 1:0 (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67340 targets N-PAC in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen N-PAC fusion protein Ag7133 相同性解析による交差性が予測される生物種
Full Name cytokine-like nuclear factor n-pac
Calculated molecular weight 61 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC064940
Gene symbol N-PAC
Gene ID (NCBI) 84656
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 N-PAC antibody CL488-67340Download protocol
FC protocol for CL Plus 488 N-PAC antibody CL488-67340Download protocol
Standard Protocols
Click here to view our Standard Protocols