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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

N4BP3 Recombinant antibody

N4BP3 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230547D12

Cat no : 83178-2-RR

Synonyms

NEDD4-binding protein 3, Nedd4 binding protein 3, KIAA0341, 230547D12

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using N4BP3 Recombinant antibody (83178-2-RR)

    WB analysis of MCF-7 using 83178-2-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83178-2-RR (N4BP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using N4BP3 Recombinant antibody (83178-2-RR)

    IHC staining of human liver using 83178-2-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83178-2-RR (N4BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using N4BP3 Recombinant antibody (83178-2-RR)

    IHC staining of human liver using 83178-2-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83178-2-RR (N4BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of MCF-7 cells using N4BP3 Recombinant antibody (83178-2-RR)

    FC experiment of MCF-7 using 83178-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug N4BP3 Recombinant antibody (83178-2-RR, Clone:230547D12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83178-2-RR

    Affinity and Kinetic Characterization of 83178-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83178-2-RR against Human N4BP3 were performed. The affinity constant is 66.3 pM.

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Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83178-2-RR targets N4BP3 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen N4BP3 fusion protein Ag10194 相同性解析による交差性が予測される生物種
Full Name Nedd4 binding protein 3
Calculated molecular weight 544 aa, 60 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC053323
Gene symbol N4BP3
Gene ID (NCBI) 23138
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

N4BP3, also named as KIAA0341, a Fezzin family member, during nerve cell development. It is crucial for axonal and dendritic branching in developing neurons. It binds the protein NEDD4 and interacts with 14-3-3 proteins. N4BP3 is present in early neurites, growth cones, axons and dendrites of developing primary hippocampal neurons.

Protocols

Product Specific Protocols
WB protocol for N4BP3 antibody 83178-2-RRDownload protocol
IHC protocol for N4BP3 antibody 83178-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols