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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated N4BP3 Recombinant an (CL750-83178-2)

    WB analysis using CL750-83178-2

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83178-2 (N4BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 750-conjugated N4BP3 Recombinant an (CL750-83178-2)

    FC experiment of MCF-7 using CL750-83178-2

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Coralite® Plus 750 N4bp3 Recombinant Antibody (CL750-83178-2, Clone:230547D12)(red), or 0.8 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite® Plus 750-conjugated N4BP3 Recombinant antibody

N4BP3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-83178-2
Clone No.230547D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra)

Synonyms

NEDD4-binding protein 3, Nedd4 binding protein 3, KIAA0341, 230547D12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-83178-2 targets N4BP3 in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen N4BP3 fusion protein Ag10194 相同性解析による交差性が予測される生物種
Full Name Nedd4 binding protein 3
Calculated molecular weight 544 aa, 60 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC053323
Gene Symbol N4BP3
Gene ID (NCBI) 23138
RRIDAB_3673776
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15049
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

N4BP3, also named as KIAA0341, a Fezzin family member, during nerve cell development. It is crucial for axonal and dendritic branching in developing neurons. It binds the protein NEDD4 and interacts with 14-3-3 proteins. N4BP3 is present in early neurites, growth cones, axons and dendrites of developing primary hippocampal neurons.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 N4BP3 antibody CL750-83178-2Download protocol
FC protocol for CL Plus 750 N4BP3 antibody CL750-83178-2Download protocol
Standard Protocols
Click here to view our Standard Protocols