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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 555-conjugated NANOG Polyclonal antibody

NANOG Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-14295

Synonyms

hNanog

Validation Data Gallery

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  • Flow cytometry (FC) experiment of NCCIT cells using CoraLite® Plus 555-conjugated NANOG Polyclonal ant (CL555-14295)

    FC experiment of NCCIT using CL555-14295

    1X10^6 NCCIT cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human NANOG (CL555-14295) (red), or 0.2 ug CoraLite® Plus 555 Rabbit IgG Control Antibody(CL555-30000)(Blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inNCCIT cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-14295 targets NANOG in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NANOG fusion protein Ag5645 相同性解析による交差性が予測される生物種
Full Name Nanog homeobox
Calculated molecular weight 35 kDa
Observed molecular weight35-40 kDa
GenBank accession numberBC160187
Gene symbol NANOG
Gene ID (NCBI) 79923
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is post-translation modified form (~48kd) (21136380 ). Nanog exists two isoforms with molecular weight 34.4 kDa and 31.9 kDa. (PMID: 21969378)

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 NANOG antibody CL555-14295Download protocol
Standard Protocols
Click here to view our Standard Protocols