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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAP1L4 Polyclonal antibody (27889-1-AP)

    WB analysis using 27889-1-AP (same clone as 27889-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27889-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27889-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NAP1L4 Polyclonal antibody (27889-1-AP)

    WB analysis of HeLa using 27889-1-AP (same clone as 27889-1-PBS)

    WB result of NAP1L4 antibody (27889-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAP1L4 transfected HeLa cells. This data was developed using the same antibody clone with 27889-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using NAP1L4 Polyclonal antibody (27889-1-AP)

    WB analysis of human testis using 27889-1-AP (same clone as 27889-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 27889-1-AP (NAP1L4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27889-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

NAP1L4 Polyclonal antibody, PBS Only

NAP1L4 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 27889-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27889-1-PBS targets NAP1L4 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAP1L4 fusion protein Ag27469 相同性解析による交差性が予測される生物種
Full Name nucleosome assembly protein 1-like 4
Calculated molecular weight 375 aa, 43 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC022090
Gene Symbol NAP1L4
Gene ID (NCBI) 4676
RRIDAB_2881006
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99733
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NAP1L4 is a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer.