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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAPRT1 Monoclonal antibody (66159-1-Ig)

    WB analysis using 66159-1-Ig (same clone as 66159-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NAPRT1 Monoclonal antibody (66159-1-Ig)

    WB analysis using 66159-1-Ig (same clone as 66159-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NAPRT1 Monoclonal antibody (66159-1-Ig)

    WB analysis of HepG2 cells using 66159-1-Ig (same clone as 66159-1-PBS)

    WB result of NAPRT1 antibody (66159-1-Ig, 1:6000) with si-Control and si-NAPRT1 transfected HepG2 cells. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NAPRT1 Monoclonal antibody (66159-1-Ig)

    WB analysis of HEK-293 using 66159-1-Ig (same clone as 66159-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NAPRT1 Monoclonal antibody (66159-1-Ig)

    WB analysis of HepG2 using 66159-1-Ig (same clone as 66159-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using NAPRT1 Monoclonal antibody (66159-1-Ig)

    IHC staining of human small intestine using 66159-1-Ig (same clone as 66159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using NAPRT1 Monoclonal antibody (66159-1-Ig)

    IHC staining of human small intestine using 66159-1-Ig (same clone as 66159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NAPRT1 Monoclonal antibody (66159-1-Ig)

    IF Staining of HeLa using 66159-1-Ig (same clone as 66159-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66159-1-Ig(NAPRT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NAPRT1 Monoclonal antibody (66159-1-Ig)

    IF Staining of HeLa using 66159-1-Ig (same clone as 66159-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAPRT1 antibody (66159-1-Ig, Clone: 5D8H10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NAPRT1 Monoclonal antibody (66159-1-Ig)

    IF Staining of HeLa using 66159-1-Ig (same clone as 66159-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAPRT1 antibody (66159-1-Ig, Clone: 5D8H10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

NAPRT1 Monoclonal antibody, PBS Only

NAPRT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66159-1-PBS
Clone No.5D8H10

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NAPRT, FHIP, FHA-HIT-interacting protein, FHA HIT interacting protein, EC:6.3.4.21

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66159-1-PBS targets NAPRT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NAPRT1 fusion protein Ag4265 相同性解析による交差性が予測される生物種
Full Name nicotinate phosphoribosyltransferase domain containing 1
Calculated molecular weight 514 aa, 55 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC032466
Gene Symbol NAPRT1
Gene ID (NCBI) 93100
RRIDAB_2881555
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6XQN6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).