Validation Data Gallery
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Tested Applications
| Positive WB detected in | THP-1 cells, HEK-293 cells, Jurkat cells, HepG2 cells, LNCaP cells, HeLa cells, K-562 cells |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:100-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
Product Information
66159-1-Ig targets NAPRT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NAPRT1 fusion protein Ag4265 相同性解析による交差性が予測される生物種 |
| Full Name | nicotinate phosphoribosyltransferase domain containing 1 |
| Calculated molecular weight | 514 aa, 55 kDa |
| Observed molecular weight | 51 kDa |
| GenBank accession number | BC032466 |
| Gene Symbol | NAPRT1 |
| Gene ID (NCBI) | 93100 |
| RRID | AB_2881555 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6XQN6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NAPRT1 antibody 66159-1-Ig | Download protocol |
| IHC protocol for NAPRT1 antibody 66159-1-Ig | Download protocol |
| IF protocol for NAPRT1 antibody 66159-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation. | ||
Nat Commun PPM1D mutations silence NAPRT gene expression and confer NAMPT inhibitor sensitivity in glioma. | ||
Cell Chem Biol NAMPT-targeting PROTAC and nicotinic acid co-administration elicit safe and robust anti-tumor efficacy in NAPRT-deficient pan-cancers | ||
Mol Cancer Ther Exploiting Metabolic Defects in Glioma with Nanoparticle Encapsulated NAMPT Inhibitors | ||
Mol Cancer Res NAPRT silencing in FH-deficient renal cell carcinoma confers therapeutic vulnerabilities via NAD+ depletion | ||
Eur J Med Chem Discovery of potent and novel dual NAMPT/BRD4 inhibitors for efficient treatment of hepatocellular carcinoma |