Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 66159-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis using 66159-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 cells using 66159-1-Ig
WB result of NAPRT1 antibody (66159-1-Ig, 1:6000) with si-Control and si-NAPRT1 transfected HepG2 cells.
WB analysis of HEK-293 using 66159-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 66159-1-Ig
HepG2 cells were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human small intestine using 66159-1-Ig
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human small intestine using 66159-1-Ig
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 66159-1-Ig
Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66159-1-Ig(NAPRT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of HeLa using 66159-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAPRT1 antibody (66159-1-Ig, Clone: 5D8H10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).
IF Staining of HeLa using 66159-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAPRT1 antibody (66159-1-Ig, Clone: 5D8H10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Featured Product
KD/KO Validated

NAPRT1 Monoclonal antibody

NAPRT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66159-1-Ig

Clone No.5D8H10

Publications(6)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NAPRT, FHIP, FHA-HIT-interacting protein, FHA HIT interacting protein, EC:6.3.4.21

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	THP-1 cells, HEK-293 cells, Jurkat cells, HepG2 cells, LNCaP cells, HeLa cells, K-562 cells
Positive IHC detected in	human small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below

Product Information

66159-1-Ig targets NAPRT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	NAPRT1 fusion protein Ag4265 相同性解析による交差性が予測される生物種
Full Name	nicotinate phosphoribosyltransferase domain containing 1
Calculated molecular weight	514 aa, 55 kDa
Observed molecular weight	51 kDa
GenBank accession number	BC032466
Gene Symbol	NAPRT1
Gene ID (NCBI)	93100
RRID	AB_2881555
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q6XQN6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).

Protocols

Product Specific Protocols
WB protocol for NAPRT1 antibody 66159-1-Ig	Download protocol
IHC protocol for NAPRT1 antibody 66159-1-Ig	Download protocol
IF protocol for NAPRT1 antibody 66159-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation. Authors - Antonella Managò View Article
human	WB	Nat Commun PPM1D mutations silence NAPRT gene expression and confer NAMPT inhibitor sensitivity in glioma. Authors - Nathan R Fons View Article
human	WB	Cell Chem Biol NAMPT-targeting PROTAC and nicotinic acid co-administration elicit safe and robust anti-tumor efficacy in NAPRT-deficient pan-cancers Authors - Xiaotong Zhu View Article
human	WB	Mol Cancer Ther Exploiting Metabolic Defects in Glioma with Nanoparticle Encapsulated NAMPT Inhibitors Authors - Matthew A Murray View Article
human	WB	Mol Cancer Res NAPRT silencing in FH-deficient renal cell carcinoma confers therapeutic vulnerabilities via NAD+ depletion Authors - Katelyn J Noronha View Article
human	WB	Eur J Med Chem Discovery of potent and novel dual NAMPT/BRD4 inhibitors for efficient treatment of hepatocellular carcinoma Authors - Chunjia Yin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 66159-1-Ig

WB analysis using 66159-1-Ig

WB analysis of HepG2 cells using 66159-1-Ig

WB analysis of HEK-293 using 66159-1-Ig

WB analysis of HepG2 using 66159-1-Ig

IHC staining of human small intestine using 66159-1-Ig

IHC staining of human small intestine using 66159-1-Ig

IF Staining of HeLa using 66159-1-Ig

IF Staining of HeLa using 66159-1-Ig

IF Staining of HeLa using 66159-1-Ig

NAPRT1 Monoclonal antibody

NAPRT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation.

PPM1D mutations silence NAPRT gene expression and confer NAMPT inhibitor sensitivity in glioma.

NAMPT-targeting PROTAC and nicotinic acid co-administration elicit safe and robust anti-tumor efficacy in NAPRT-deficient pan-cancers

Exploiting Metabolic Defects in Glioma with Nanoparticle Encapsulated NAMPT Inhibitors

NAPRT silencing in FH-deficient renal cell carcinoma confers therapeutic vulnerabilities via NAD+ depletion

Discovery of potent and novel dual NAMPT/BRD4 inhibitors for efficient treatment of hepatocellular carcinoma