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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated NAPRT1 Monoclonal an (CL488-66159)

    IF Staining of HeLa using CL488-66159

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 NAPRT1 antibody (CL488-66159, Clone: 5D8H10 ) at dilution of 1:200.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated NAPRT1 Monoclonal antibody

NAPRT1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66159
Clone No.5D8H10

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

FHA HIT interacting protein, FHIP, NAPRT1, NAPRTase, PP3856

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66159 targets NAPRT1 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NAPRT1 fusion protein Ag4265 相同性解析による交差性が予測される生物種
Full Name nicotinate phosphoribosyltransferase domain containing 1
Calculated molecular weight 514 aa, 55 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC032466
Gene Symbol NAPRT1
Gene ID (NCBI) 93100
RRIDAB_2883274
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6XQN6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NAPRT1 antibody CL488-66159Download protocol
Standard Protocols
Click here to view our Standard Protocols