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Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using NARF Polyclonal antibody (12173-1-AP)

    WB analysis of human liver using 12173-1-AP (same clone as 12173-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12173-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using NARF Polyclonal antibody (12173-1-AP)

    WB analysis of human placenta using 12173-1-AP (same clone as 12173-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12173-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using NARF Polyclonal antibody (12173-1-AP)

    WB analysis of mouse testis using 12173-1-AP (same clone as 12173-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12173-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human lung tissue using NARF Polyclonal antibody (12173-1-AP)

    WB analysis of human lung using 12173-1-AP (same clone as 12173-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12173-1-PBS in a different storage buffer formulation.

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NARF Polyclonal antibody, PBS Only

NARF Polyclonal Antibody for WB, Indirect ELISA
Cat No. 12173-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12173-1-PBS targets NARF in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NARF fusion protein Ag2816 相同性解析による交差性が予測される生物種
Full Name nuclear prelamin A recognition factor
Calculated molecular weight 46 kDa, 53 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC016440
Gene Symbol NARF
Gene ID (NCBI) 26502
RRIDAB_2151027
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHQ1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Prelamin A, is processed in the nucleus to lamin A by removal of its final 18 amino acids, including the cysteine residue in its C-terminal CAAX box, which is farnesylated. Nuclear prelamin A recognition factor (NARF) binds the farnesylated prelamin A C-terminal domain(preAct)(PMID:10514485). NARF, at 52 kDa, also named as IOP2, is a small enough protein to freely diffuse through the nuclear pore complex and may be retained within the nucleus by binding nuclear proteins and it has all of the information necessary to be imported into the nucleus(PMID:10514485). It has 3 isoforms produced by alternative splicing with the molecular mass of 51 kDa, 56 kDa and 46 kDa,respectively.