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NAT10 Polyclonal antibody
NAT10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 13365-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, NIH/3T3 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13365-1-AP targets NAT10 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NAT10 fusion protein Ag4184 相同性解析による交差性が予測される生物種 |
| Full Name | N-acetyltransferase 10 (GCN5-related) |
| Calculated molecular weight | 1025 aa, 116 kDa |
| Observed molecular weight | 116 kDa |
| GenBank accession number | BC035558 |
| Gene symbol | NAT10 |
| Gene ID (NCBI) | 55226 |
| RRID | AB_2148944 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA. (24786082)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NAT10 antibody 13365-1-AP | Download protocol |
| IHC protocol for NAT10 antibody 13365-1-AP | Download protocol |
| IF protocol for NAT10 antibody 13365-1-AP | Download protocol |
| IP protocol for NAT10 antibody 13365-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Host Microbe Acetylation of Cytidine Residues Boosts HIV-1 Gene Expression by Increasing Viral RNA Stability.
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Mol Cell Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine. | ||
Nat Commun Targeting of NAT10 enhances healthspan in a mouse model of human accelerated aging syndrome.
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Nucleic Acids Res NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH SONAM (Verified Customer) (12-18-2023) | Antibody works really well. No Nonspecific bindings.
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FH Mattia (Verified Customer) (09-12-2019) | Clear band at the right size, when antibody used at 1:1500 dilution in 5% milk an incubated at 2hrs at 25C. Band is a bit streaky, may require further optimisation.
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FH Jonathan (Verified Customer) (11-29-2018) |
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