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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated NAT10 Polyclonal ant (CL488-13365)

    IF Staining of HeLa using CL488-13365

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 NAT10 antibody (CL488-13365) at dilution of 1:200.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite® Plus 488-conjugated NAT10 Polyclonal ant (CL488-13365)

    FC experiment of NIH/3T3 using CL488-13365

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated NAT10 Polyclonal antibody (CL488-13365)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  • KD/KO Validated

CoraLite® Plus 488-conjugated NAT10 Polyclonal antibody

NAT10 Polyclonal Antibody for IF/ICC
Cat No. CL488-13365

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

N-acetyltransferase 10, N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13365 targets NAT10 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAT10 fusion protein Ag4184 相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 10 (GCN5-related)
Calculated molecular weight 1025 aa, 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC035558
Gene Symbol NAT10
Gene ID (NCBI) 55226
RRIDAB_3083938
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0A0
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA. (24786082)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NAT10 antibody CL488-13365Download protocol
Standard Protocols
Click here to view our Standard Protocols